Italy defender Alessandro Nesta pulled out of a training session on Wednesday ahead of his team's decisive World Cup group G match against Mexico.
Nesta had been suffering from a bruised right foot picked up in Saturday's 2-1 defeat to Croatia.
The central defender took part in a light warm-up session and played five minutes of a practice match but after discussions with coach Giovanni Trapattoni he left the field with a member of the coaching staff.
Nesta was not limping but it was clearly not good news for Trapattoni that his key defender was unable to complete the training.
Midfielder Cristiano Zanetti, who had also been in discomfort due to a foot injury, did take part in the training session and appeared to be fine.
Luigi Di Biagio, ruled out for 15 days with a calf injury, was the only player missing from the training session.
Italy must beat Mexico to be sure of taking their place in the second round.